S6022

Sephadex^® G-50

BioReagent, DNA grade, for molecular biology, medium

• CAS Number: 9004-54-0
• MDL number: MFCD00132771
• UNSPSC Code: 23151817
• NACRES: NA.52

Properties

• grade: Molecular Biology; for molecular biology
• product line: BioReagent
• form: beads
• swelling: 1 g swells to 9-11 mL
• bead diameter: 50-150 μm
• pore size: exclusion limit (20 bp dsDNA; (rA)₂₀)
• pH: 2-10
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• SMILES string: O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
• InChI: 1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
• InChI key: FZWBNHMXJMCXLU-UHFFFAOYSA-N

Description

General description

Sephadex^® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex^® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.[1][2]

Sephadex^® G-50 has been used to purify the DNA products in cycle sequencing reactions.[3][2]

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves