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General description
Sigma′s GenElute™ FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Sigma′s GenElute™ FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.
- Fast and easy processing using rapid spin-column format
- High yields and Quality of Nucleic Acids
- Separate fractionation of RNA and DNA
- Isolate total RNA, from large rRNA down to microRNA (miRNA)
- No phenol or chloroform extractions
Application
RNA
Genomic DNA
- Bioanalyzer
- Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
- RT-PCR for both large mRNA and small RNA including miRNA
- Northern blotting
- RNase protection
- Primer extension
- Expression array assays
- Next Generation Sequencing
- microRNA Cloning
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
Genomic DNA
- PCR and Quantitative PCR
- Sequencing
- DNA Methylation Studies
- Southern blotting
- Microarrays
- SNP analysis
Features and Benefits
- Complete column purification - Separate fractionation of RNA and DNA
- Reduce variability - RNA and DNA are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
- Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
- Isolate high quality RNA and DNA - The purified RNA and DNA are of the highest quality and can be used in a number of downstream applications
Unit Definition
Preps
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
8A - Combustible corrosive hazardous materials
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