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RAB0747

Sigma-Aldrich

Human TF / Serotransferrin ELISA Kit

Synonym(s):

Serotransferrin, TF, Transferrin

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 ng/mL
standard curve range: 1.029-750 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TF(7018)

General description

Transferrin (TF) is an iron (Fe) transport protein and found in blood plasma and brain extracellular fluids. It belongs to the family of Fe-binding glycoproteins. TF is made of a single polypeptide chain with two domains and each domain consists of a Fe-binding site. It helps in the transport of Fe(III) between the cells in soluble and non-toxic forms. TF also acts as a bacteriostatic agent as well as a growth factor. It binds to the transferrin receptor to mediate the cellular uptake of bound Fe. The TF gene is mapped on the human chromosome at 3q22.1. The antibody pair provided in this kit recognizes human serotransferrin.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Trace elements, like Cu, Zn, Fe, or Se, are important for the proper functioning of antioxidant enzymes. However, in excessive amounts, they can also act as pro-oxidants. Accordingly, trace elements influence redox-modulated signaling pathways, such as the Nrf2 pathway. Vice
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Niemann-Pick C1 (NPC1) is a lysosomal cholesterol storage disorder, that severely affects the brain, and is caused by mutations in the NPC1 gene, which encodes an intracellular membrane transporter of non-esterified cholesterol. Therapeutic options for NPC1 are few, and classical
T Moos et al.
Cellular and molecular neurobiology, 20(1), 77-95 (2000-02-26)
1. Iron (Fe) is an essential component of virtually all types of cells and organisms. In plasma and interstitial fluids, Fe is carried by transferrin. Iron-containing transferrin has a high affinity for the transferrin receptor, which is present on all
Stefanie Dichtl et al.
mBio, 10(1) (2019-02-07)
We have recently shown that the catecholamine dopamine regulates cellular iron homeostasis in macrophages. As iron is an essential nutrient for microbes, and intracellular iron availability affects the growth of intracellular bacteria, we studied whether dopamine administration impacts the course
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