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RAB0188

Sigma-Aldrich

Human FGF-7 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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1 KIT
€608.00

€608.00

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1 KIT
€608.00

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

€608.00

Please contact Customer Service for Availability

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.55-400 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF7(2252)

General description

The Human FGF-7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF-7 in serum, plasma, cell culture supernatants and urine.
The gene FGF7 (fibroblast growth factor 7) is mapped to human chromosome 15q21.1.[1] The encoded protein belongs to the fibroblast growth factor family and is secreted by mesenchymal cells.[2][3]

Immunogen

Recombinant Human FGF7

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

FGF7 (fibroblast growth factor 7) is involved in the development of skin and sweat glands, wound healing and cutaneous homeostasis.[4] It controls keratinocyte differentiation and viability by modulating autophagy.[2] FGF7 also participates in embryonic development, tumorigenesis, cell proliferation, migration and differentiation.[3] It is linked with several disorders like nonsyndromic cleft lip and palate, thyroid volume, goiter risk and chronic obstructive pulmonary disease. FGF7 plays an important role in the morphology of developing lungs. Absence of the FGF7 activity decreases airway branching during development. During injury, it is involved in airway repair.[5]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
1005J0245
0922J0245
0324J0245
0803I0245
0706I0245

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Association between fibroblast growth factor 7 and the risk of chronic obstructive pulmonary disease
Xu SC, et al.
Acta Pharmacologica Sinica, 33(8), 998-1003 (2012)
FGF7 Over Expression is an Independent Prognosticator in Patients with Urothelial Carcinoma of the Upper Urinary Tract and Bladder
Fan EW, et al.
The Journal of Urology, 194(1), 223-229 (2015)
Recombinant keratinocyte growth factor 1 in tobacco potentially promotes wound healing in diabetic rats
Feng ZG, et al.
BioMed Research International (2014)
Oncogenic mutations of ALK kinase in neuroblastoma
Chen Y, et al.
Nature, 455(7215), 971-974 (2008)
Role of Keratinocyte Growth Factor in the Differentiation of Sweat Gland-Like Cells From Human Umbilical Cord-Derived Mesenchymal Stem Cells.
Xu Y
Stem Cells Translational Medicine, 5(1), 106-116 (2016)

Questions

1–3 of 3 Questions  

  1. What is the specificity of the Human FGF7 ELISA antibodies (RAB0188)?

    1 answer

    1. The capture antibody utilized in this kit is a mouse monoclonal antibody with an immunogen of aa32-194 of human FGF-7 (accession # P21781). Meanwhile, the detection antibody is a goat polyclonal antibody with the immunogen of aa32-194 of human FGF-7 (accession # Q6FGV5).

      Helpful?

  2. Will the Human FGF-7 ELISA kit (BAB0188) detect mouse FGF7?

    1 answer

    1. The Human FGF-7 ELISA kit has not been tested for cross-reactivity to mouse, so we cannot confirm if it can detect mouse FGF-7. However, we do offer a Mouse FGF-7 ELISA with the catalog number RAB1123.

      Helpful?

  3. Is there a detailed protocol for this (the general sandwich protocol is not specific enough)? E.g. the kit FGF-7 concentrate for the standard curve - no concentration is written on the vial. And there is no information on how to make serial dilutions.

    1 answer

    1. Using the lot number on the product you purchased, you can access the lot-specific CofA to view the procedure and concentration. Example CofA using lot number 0922J0245: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0188_0922J0245.pdf

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