MAK114

Glyoxalase I Activity Assay Kit

sufficient for 100 tests (UV)

• Synonym(s): Aldoketomutase Activity Assay, GLO1 Activity Assay, Ketone-Aldehyde Mutase, Activity Assa, Lactoylglutathione Lyase Activity Assay, Methylglyoxalase Activity Assay
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 tests (UV)
• detection method: colorimetric
• relevant disease(s): cancer
• storage temp.: −20°C
• Gene Information: human ... GLO1(2739) ; mouse ... GLO1(109801) ; rat ... GLO1(294320)

Description

General description

Glyoxalase I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methyglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans, and cancer.

Application

Glyoxalase I Assay Kit has been used to estimate the glyoxalase I activity in plasma samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of Glyoxalase I activity in enzyme preparations and biological samples. Suitable for testing the effects of compounds on Glyoxalase I activity.

Principle

The Glyoxalase I Activity Assay kit provides a simple and direct procedure for measuring GLO-1 activity in a variety of samples such as enzyme preparations or biological samples. In this assay, the GLO-1-mediated production of S-lactoylglutathione is measured by monitoring the change in absorbance at 240 nm. One unit of Glyoxalase I is the amount of enzyme that will convert 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.6 and 25 °C.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319,H341
• Precautionary Statements: P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids