SCL 4.1/F7
93031204, rat nerve (sheath), Flattened or crescent shaped
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About This Item
UNSPSC Code:
41106514
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Product Name
SCL 4.1/F7, 93031204
biological source
rat nerve (sheath)
growth mode
Adherent
karyotype
42,XY / 43,XY+7
morphology
Flattened or crescent shaped
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Schwann cell
Cell Line Description
This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated (stellate / bipolar) morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at ECACC. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.
Culture Medium
Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C Saturation density is 10,000 cells/cm2.
Other Notes
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