biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-IgG2a, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Cell Line Description
The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. The IgM antibodies bind murine IgG2a of "a" allotype and Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.
Immunogen
A/PR/8/34 mouse pathogenic human influenza virus
Application
Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.
Culture Medium
RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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