product name
HSDM1C1, 90112810
biological source
mouse fibrosarcoma
growth mode
Adherent
karyotype
2n = 40
morphology
Fibroblast
products
Prostaglandin E2
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Swiss Albino fibrosarcoma
Cell Line Description
A clone derived from the parental HSDM1 cell line established from a transplantable fibrosarcoma of a Swiss Albino mouse. The cells secrete prostaglandin E2 which is inhibited by aspirin like drugs such as indomethacin.
Application
Hormone studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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