AFRC MAC 9
85102271, Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii
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About This Item
UNSPSC Code:
41106514
biological source
Rat x Rat Hybridoma
ImmunoDon
AO rat spleen
Myeloma
YB2/3.0 Ag20
Reactivity
Anti-Glycoprotein of Chlamydomonas reinhardii
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat YB2/3.0 Ag20 x Rat AO spleen
Cell Line Description
The antibody recognises the glycoprotein 2BII of Chlamydomonas reinhardii. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 9 shows similar specificity and properties to AFRC MAC 6, 7, 8, 10 and 11.
Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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Certificates of Analysis (COA)
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