MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)

14050803, mouse embryo, Fibroblast

• UNSPSC Code: 41106514
• biological source: mouse embryo
• growth mode: Adherent
• morphology: Fibroblast

Properties

• Product Name: MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM), from mouse embryo, 14050803
• biological source: mouse embryo
• description: Mouse embryonic fibroblast, autophagy, Ulk1 -/- Ulk2 -/-, double knockout, spontaneously immortalised
• growth mode: Adherent
• morphology: Fibroblast
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Primary mouse embryonic fibroblasts (MEFs) were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13.5 post coitus. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by a standard serial passaging protocol.

Culture Medium

DMEM + 10% FCS + 2mM Glutamine

Subculture Routine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10,000 cells per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37^oC

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.