KARPAS-929
06072606, human bone marrow, round to polygonal cells growing in clusters in suspension
Synonym(s):
KARPAS 929, KARPAS, 929, KARPAS929
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About This Item
UNSPSC Code:
41106514
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product name
KARPAS-929, from human bone marrow, 06072606
biological source
human bone marrow
growth mode
Suspension
karyotype
Hyperdiploid with complex aberrant chromosomes
morphology
Round to polygonal cells growing in clusters in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Line Origin
Human multiple myeloma
Cell Line Description
Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.
DNA Profile
Not specified
Culture Medium
RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)
Subculture Routine
Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105 cells/ml, this tends to equate to a 1:3 split every 2-3 days.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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