Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

A3653

Sigma-Aldrich

Actin from bovine muscle

lyophilized powder

Synonym(s):

G-Actin

Sign Into View Organizational & Contract Pricing

Select a Size

5 MG
€576.00

€576.00

Please contact Customer Service for Availability
All Photos(1)

Select a Size

Change View
5 MG
€576.00

About This Item

CAS Number:
51005-14-2
MDL number:
MFCD00130338
UNSPSC Code:
12352202
NACRES:
NA.32

€576.00

Please contact Customer Service for Availability

biological source

bovine muscle

Quality Level

200

Assay

≥90% (SDS-GE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

western blot: suitable

UniProt accession no.

P62739

storage temp.

2-8°C

Gene Information

bovine ... LOC(528168)

Looking for similar products? Visit Product Comparison Guide

General description

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.[1]

Application

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.[2][3]

Biochem/physiol Actions

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction.[1] The protein acts as a building block of microfilaments.[4]

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLBX5919
SLCB8061
SLBQ7282V
SLBM5951V
SLBD7277V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Rheumatoid synovial fibroblasts constitutively express the fibrinolytic pattern of invasive tumor-like cells
Guiducci S, et al.
Clinical and Experimental Rheumatology, 23(3), 364-372 (2005)
Laure Diancourt et al.
Toxins, 11(3) (2019-03-03)
Clostridiumperfringens type E is a less frequently isolated C.perfringens type and has not previously been reported in France. We have characterized two recent type E isolates, C.perfringens 508.17 from the intestinal content of a calf that died of enterotoxemia, and
Zsuzsanna Kolbert et al.
Plant & cell physiology, 59(9), 1827-1843 (2018-05-26)
At high concentrations, selenium (Se) exerts phytotoxic effects in non-tolerant plant species partly due to the induction of nitro-oxidative stress; however, these processes are not fully understood. In order to obtain a more accurate view of the involvement of nitro-oxidative
T V L Berghof et al.
Poultry science, 89(2), 227-235 (2010-01-16)
Natural antibodies (NAb) have been divided in 2 classes: overt and cryptic. Overt NAb can be detected in unfractionated normal sera of nonimmunized mammals and chickens. Cryptic NAb as described in mammals need an in vitro physical or biochemical treatment
Contrasting cellular stress responses of Baikalian and Palearctic amphipods upon exposure to humic substances: environmental implications
Protopopova MV, et al.
Environmental Science and Pollution Research International, 21(24), 14124-14137 (2014)
View All Related Papers

Articles

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

Questions

  1. Can you provide the protocol to polymerize the Actin from Bovine muscle?

    1 answer

    1. In the absence of salts, actin exists as a globular monomeric protein (G-actin). In the presence of ATP, a divalent cation (Ca2+ or Mg2+) and high salts (KCl, NaCl or MgCl2), the G-actin polymerizes to form a fibrous or filamentous actin (F-actin) which incorporates the cation and ATP (3,4). Dialysis of the F-actin solution (viscous) against water reverses the polymerization (2). The actin as it is supplied in product A3653 has been extensively dialyzed to be converted into G-Actin.

      This is described in the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/659/a3653pis.pdf

      References
      2. Sigma quality control and production.
      3. Carlier, M.-F., J. Biol. Chem., 266, 1-4 (1991) - citing Kabsch, W. et al., Nature, 347, 37-44 (1990).
      4. Merck Index, 12th Ed., #137 (1996).

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service