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A0802

Sigma-Aldrich

Adipic acid dihydrazide–Agarose

saline suspension

Synonym(s):

Adipic acid dihydrazide agarose beads

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10 ML
€265.00
50 ML
€1,030.00

€265.00

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10 ML
€265.00
50 ML
€1,030.00

About This Item

MDL number:
MFCD00161954
UNSPSC Code:
23151817
NACRES:
NA.56

€265.00

Please contact Customer Service for Availability

biological source

plant

form

saline suspension

extent of labeling

≥8 μmol per mL

technique(s)

microbe id | metabolite detection: suitable

matrix activation

cyanogen bromide

matrix spacer

11 atoms (when ligands (aldehydes, carboxylic acids) are coupled through free hydrazide groups)

suitability

suitable for chromatography

storage temp.

2-8°C

Related Categories

Application

Adipic acid dihydrazide Agarose can be used in proteomics and protein chromatography. It has been utilized in research for purifying and identifying the fungal phytotoxin Fusicoccin (FC), identifying RNA binding proteins that interact with RNA cis-elements, and enzyme purification such as peroxisomes from guinea pig liver.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000375834
0000348935
0000348935
0000375834
SLCJ3402

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Ruben Hovhannisyan et al.
BioTechniques, 46(2), 95-98 (2009-03-26)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity in cellular extracts that can degrade
M Caputi et al.
The EMBO journal, 18(14), 4060-4067 (1999-07-16)
Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are
S C Datta et al.
The Journal of biological chemistry, 265(14), 8268-8274 (1990-05-15)
The peroxisomal acyl/alkyl dihydroxyacetone-phosphate reductase (EC 1.1.1.101) was solubilized and purified 5500-fold from guinea pig liver. The enzyme could be solubilized by detergents only at high ionic strengths in presence of the cosubstrate NADPH. Peroxisomes, isolated from liver by a
Abrahem Abrahem et al.
Nucleic acids research, 36(16), 5201-5211 (2008-08-07)
Although RNA polymerases (RNAPs) are able to use RNA as template, it is unknown how they recognize RNA promoters. In this study, we used an RNA fragment derived from the hepatitis delta virus (HDV) genome as a model to investigate
M Caputi et al.
The Journal of biological chemistry, 276(47), 43850-43859 (2001-09-26)
Members of the heterogeneous nuclear ribonucleoprotein (hnRNP) H protein family, H, H', F, and 2H9, are involved in pre-mRNA processing. We analyzed the assembly of these proteins from splicing extracts onto four RNA regulatory elements as follows: a high affinity
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