77958
Abberior®CAGE 590, NHS-ester
ideal for single-molecule siwtching microscopy (e.g. PALM, STORM, GSDIM)
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About This Item
UNSPSC Code:
12352108
NACRES:
NA.32
Recommended Products
form
solid
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 590 nm; λem 600-620 nm in PBS, pH 7
storage temp.
−20°C
General description
Abberior CAGE 590 is a masked dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye. The dye opens up the red fluorescent regime for CAGE dyes. It can most effectively be excited from 560 to 600nm.
Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyse molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Key Features
Absorption Maximum, λmax: 262, 325, 351 nm (non-activated, PBS, pH 7.4)
595 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 615 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 685 −715 nm
Fluorescence Quantum Yield, η: 0.80 (after photoactivation, PBS, pH 7.47)
Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyse molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Key Features
- First CAGE dye in the red fluorescent range
- Ideal for PALM, STORM, GSDIM
- Well suited as 2nd STED color (after uncaging)
Absorption Maximum, λmax: 262, 325, 351 nm (non-activated, PBS, pH 7.4)
595 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 615 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 685 −715 nm
Fluorescence Quantum Yield, η: 0.80 (after photoactivation, PBS, pH 7.47)
Application
Abberior® CAGE 590 anti-rabbit antibody has been used as a secondary antibody for spectral-FPALM (fluorescence photo-activation localization microscopy) imaging of NIH-3T3 cells.
Suitability
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
degree of coupling: report results
Other Notes
For more information on Abberior® Dyes, please visit our overview.
Legal Information
abberior is a registered trademark of Abberior GmbH
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Michael J Mlodzianoski et al.
PloS one, 11(3), e0147506-e0147506 (2016-03-24)
Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each
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