The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol). Some people are very sensitive to the odor of 2-mercaptoethanol.
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5 ML
€39.50
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€75.70
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5 ML
€39.50
5 X 2 ML
€75.70
About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
Quality Level
form
liquid
composition
bromophenol blue, 0.004%
DTT, 400 mM
glycerol, 20%
SDS, 4%
TRIS, 0.125 M
pH
~6.8
suitability
in accordance for electrophoresis test
storage temp.
−20°C
Application
Laemmli Lysis-buffer is an non-smelling lysis buffer used to prepare protein samples for SDS gel electrophoresis.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can
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Tissue-dependent oestrogenic and anti-oestrogenic activity of polycyclic aromatic hydrocarbons (PAHs) has been suggested. In this study, the effect of two PAH mixtures, M1 composed of all 16 priority pollutants and M2 composed of five (noted in the highest levels) compounds
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What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?
1 answer-
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What is the difference between the two Laemmli buffers S3401 and 38733?
1 answer-
Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue. So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).
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Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?
1 answer-
An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?
1 answer-
For most applications, it is considered to be a 2X solution.
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