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08014

Sigma-Aldrich

Micro particles, magnetic, streptavidin coated

10 μm particle size, std dev <0.5 μm

Synonym(s):

Polystyrene beads, magnetic, streptavidin coated

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5 ML
€583.10
10 ML
€967.50

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5 ML
€583.10
10 ML
€967.50

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

€583.10

List Price€833.00Save 30%
Please contact Customer Service for Availability
Request a Bulk Order

form

aqueous solution

Quality Level

100

composition

iron oxide, ≥20%
iron oxide, ≥20%

concentration

1% (solids)

particle size

10 μm std dev <0.5 μm

storage temp.

2-8°C

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General description

Micro Particles or Latex Beads, commonly known as polystyrene microparticles, are negatively charged colloidal particles. The microparticles are produced through a polymerization reaction of styrene under controlled conditions. The conducive environment of the reaction induces spontaneous coalescent bead formation. The biotin-streptavidin system is the strongest noncovalent biological interaction known, having a dissociation constant, K(d), in the order of 4x10(-14) M.[1] The beads are magnetic in nature and coated with streptavidin for enhanced binding. Micro Particles or Latex beads are supplied in aqueous solutions composed of polymer particles with water. Polystyrene′s refractive index at 589 nm is 1.5905 and 1.602 at 486 nm.
Superparamagnetic polystyrene particles

Application

Easily conjugated with biotin, making them useful for several detection assays,.
Micro Particles or Latex Beads is used for a wide variety of applications like:
  • Electron Microscopy.
  • Cell Counter Calibration.
  • Immunodiagnostics, especially for latex agglutination tests, and
  • Phagocytosis Research.
Microparticles with streptavidin are easily conjugated with biotin for use in various detection assays

Analysis Note

Binding capacity for FITC-Biotin: >300 pmol/mg

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Anders Holmberg et al.
Electrophoresis, 26(3), 501-510 (2005-02-04)
The biotin-streptavidin system is the strongest noncovalent biological interaction known, having a dissociation constant, K(d), in the order of 4x10(-14) M. The strength and specificity of the interaction has led it to be one of the most widely used affinity
Rongrong Huang et al.
Autophagy, 13(10), 1722-1741 (2017-08-09)
Circular RNAs are a subclass of noncoding RNAs in mammalian cells; however, whether these RNAs are involved in the regulation of astrocyte activation is largely unknown. Here, we have shown that the circular RNA HIPK2 (circHIPK2) functions as an endogenous

Questions

1–2 of 2 Questions  

  1. What is the concentration of the beads (number/ml)?

    1 answer

    1. The estimated particle concentration is 1.3 x 10^7 per milliliter. This is a calculated value based on the following specifications:
      Polymer density: 1.47 g/cc
      Mean particle diameter: 10 um
      Particle concentration in percentage of aq. suspension: 10 mg/ml (1%).

      Helpful?

  2. Could you reveal the buffer composition for the buffer that the beads are supplied in?

    1 answer

    1. This product is prepared in water rather than a buffer solution. No surfactant has been added.

      Helpful?

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