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HKG6PDH-RO

Roche

Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)

from yeast/Leuconostoc, overproducer

Synonym(s):

G6P-DH, Glucose-6-Phosphate DehydrogenaseHK, Hexokinase

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About This Item

UNSPSC Code:
12352204

biological source

yeast

Quality Level

100

form

suspension

mol wt

dimer 110,000 Da

packaging

pkg of 10 mL (10737275001 [30 mg])
pkg of 5 mL (10127825001 [15 mg])

manufacturer/tradename

Roche

concentration

3.21 mg/mL

technique(s)

activity assay: suitable

color

white

pH

5.5-6.5

solubility

water: miscible

suitability

suitable for UV spectrophotometry and general use

application(s)

life science and biopharma

storage temp.

2-8°C

General description

Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.

Application

Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.

Components

EC 2.7.1.1 & 1.1.1.49

Unit Definition

One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.

Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.

Other Notes

For life science research only. Not for use in diagnostic prodcedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Pelicano et al.
Oncogene, 25(34), 4633-4646 (2006-08-08)
Most cancer cells exhibit increased glycolysis and use this metabolic pathway for generation of ATP as a main source of their energy supply. This phenomenon is known as the Warburg effect and is considered as one of the most fundamental
Miguel Vázquez-Velasco et al.
Nutricion hospitalaria, 32(6), 2718-2724 (2015-12-17)
Type 2 diabetes is a very prevalent chronic disease. Among dietary factors for its prevention and treatment, interest has grown in satiating fibre (konjac glucomannan) and spirulina. Our previous studies suggest that glucomannan itself and/or in conjunction to spirulina displayed
K Gottlob et al.
Genes & development, 15(11), 1406-1418 (2001-06-08)
The serine/threonine kinase Akt/PKB is a major downstream effector of growth factor-mediated cell survival. Activated Akt, like Bcl-2 and Bcl-xL, prevents closure of a PT pore component, the voltage-dependent anion channel (VDAC); intracellular acidification; mitochondrial hyperpolarization; and the decline in
Robert C Stanton
IUBMB life, 64(5), 362-369 (2012-03-21)
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway. Many scientists think that the roles and regulation of G6PD in physiology and pathophysiology have been well established as the enzyme was first identified 80 years ago. And

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