11363735001
Roche
Hybridoma Fusion and Cloning Supplement
solution, suitable for hybridoma, Mycoplasma, tested
Synonym(s):
HFCS
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About This Item
UNSPSC Code:
12352207
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description
HFCS
Quality Level
sterility
0.2 μm filtered
form
solution
packaging
pkg of 10 mL (50x)
manufacturer/tradename
Roche
technique(s)
cell culture | hybridoma: suitable
impurities
Mycoplasma, tested
10 EU/mL Endotoxin (LAL)
solubility
water: miscible
storage temp.
−20°C
General description
Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).
Application
Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
Physical form
Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.
Analysis Note
Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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