The 10 kDa membrane suitable for this stirred cell is PLGC07610.
UFSC40001
Amicon® Stirred Cell 400mL
This Amicon stirred cell model provides high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit.
Synonym(s):
Amicon stirred cell
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About This Item
Recommended Products
material
Nylon assembly (cap and tube fitting)
Nylon stand (retaining)
Tygon® tube (filtrate)
anodized aluminum stand (retaining)
polyacetal assembly (stirring)
polyethylene tube (pressure)
polysulfone assembly (stirring)
polysulfone holder (cylinder & membrane)
silicone O-ring
Quality Level
product line
Amicon®
manufacturer/tradename
Amicon®
parameter
400 mL process volume
technique(s)
protein clean-up: suitable (desalting)
protein purification: suitable (concentration)
H
18.7 cm (7.4 in.)
filter diam.
76 mm
filtration area
41.8 cm2
shipped in
ambient
Application
Principle
Linkage
Legal Information
Certificates of Analysis (COA)
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Articles
Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.
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Does this model have a 10 kDa membrane?
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Helpful?
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What is the minimum volume that can be concentrated using stirred cells? Additionally, what is the minimum volume that can be recovered from the stirred cell?
1 answer-
The stirred cells do not have a specified minimum concentrate volume, as this is typically dependent on the sample. According to the user guide, the minimum concentrate volume must be determined empirically by the user based on sample composition and stirring speed.
In theory, it is possible to concentrate to dryness; however, this is highly dependent on the sample and membrane used.
Additionally, the specification chart includes a hold-up volume, which indicates the volume of sample that is 'lost' to the device. For detailed hold-up volume information, please refer to page 10 of the user guide.
Helpful?
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Could you please provide more information about the troubleshooting for a stirred cell that is not holding pressure and has poor concentrate recovery?
1 answer-
On September 7, 2021, the Eyre User Guide provided troubleshooting information on pages 8 and 9. It addressed the symptom of a stirred cell not holding pressure and poor concentrate recovery. The cause identified was a membrane that was not sealed, leading to sample bypass around the membrane. The corrective action recommended was to ensure that the O-ring is free of dirt or debris and undamaged.
Helpful?
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