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SCR140

Sigma-Aldrich

Animal Free Collagenase/Dispase Blend II

Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.

Synonym(s):

MMP, Matrix metalloprotease, Dispase, Neutral Protease

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50 MG
€269.00

€269.00

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50 MG
€269.00

About This Item

UNSPSC Code:
12352204
eCl@ss:
32160405
NACRES:
NA.78

€269.00

In StockDetails

Request a Bulk Order

Quality Level

100

caseinase activity

≥2000 caseinase units/mg

clostripain activity

≤5.0 clostripain units/mg

collagenase activity

≥12,500 U/vial

trypsin activity

≤0.5 trypsin units/mg

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

Related Categories

General description

Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
Product Source: Isolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2008 certified enviroment.

Application

Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
Research Category
Stem Cell Research
Research Sub Category
Enzymes & Biochemistry

Physical form

Product is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.

Storage and Stability

Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. What should the working concentration be for cell isolation? And are there any special protocol for resuspending it? Once resuspended should it still be stored in 2-8deg C or should it be stored in -20deg C?

    1 answer

    1. This product may be reconstituted with PBS or media of choice at 1 mg/ml. The addition of Human Serum Albumin at 1% is recommended as a stabilizer. Upon reconstitution, the product solution can be sterile filtered through a 0.22 µm pore size membrane. The solution should be then aliquoted and stored frozen at -20C or -80C. It is expected to be stable for 3-6 months.

      The protocol may vary with the type of tissue, the age of the animal, or other variables. The end-user would need to adjust the temperature and incubation time, but the general recommendation is to digest at 37 deg C for 15 - 30 minutes. For protocol/concentration to be used, it may be helpful to review the literature.

      Helpful?

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