SCC119
ImKC Immortalized Mouse Kupffer Cell Line
The ImKC Murine Immortalized Kupffer Cell Line can be used to develop in vitro model cell systems to advance the study of liver injury, function, and disease.
Synonym(s):
ImKC Cell Line
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
Pricing and availability is not currently available. Contact Technical Service
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biological source
mouse
technique(s)
cell based assay: suitable
cell culture | mammalian: suitable
shipped in
ambient
General description
ImKC is a cytokine-producing immortalized Kupffer cell line that was established from transgenic mice expressing the thermolabile mutant tsA58 of the Simian virus 40 large T antigen under the control of the H-2kb promoter. Primary KC were obtained using a three step procedure: liver perfusion, centrifugal elutriation, and sorting for F4/80+ cells. ImKC were identified within the small-intermediate population of KC that maintained stable expression of F4/80, and the surface antigens CD11b, CD14 and TLR4.
Kupffer cells (KC), also known as stellate macrophages, are liver macrophages that reside in the lumen of the liver sinusoids. Kupffer cells play a critical role in maintaining liver functions including the removal of pathogens & foreign materials, antigen presentation and modulating innate immune responses. In vitro studies of Kupffer cells have been hindered by the difficulty in isolating sufficient number of primary cells and the limited lifespan of the cells in culture.
Cell Line Description
Macrophage Cells
Application
Research Category
Inflammation & Immunology
Metabolism
Inflammation & Immunology
Metabolism
Research Sub Category
Liver Physiology
Liver Physiology
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact [email protected].
Quality
• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Julia Rudolph et al.
Scientific reports, 11(1), 15702-15702 (2021-08-05)
Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial
Hsin-Tzu Liu et al.
Antioxidants (Basel, Switzerland), 11(5) (2022-05-29)
Inflammation plays an important role in the pathophysiology of depression. This study aims to elucidate the antidepressant effect of baicalein, an anti-inflammatory component of a traditional Chinese herbal medicine (Scutellaria baicalensis), on lipopolysaccharide (LPS)-induced depression-like behavior in mice, and to
Zheng-Yu Wang et al.
Cytokine, 70(2), 165-172 (2014-08-21)
Kupffer cells (KC) play a critical role in both liver physiology and the pathogenesis of various liver diseases. Isolated primary KC have a limited lifespan in culture, and due to the relatively low number obtained, limit their study in vitro.
Hepatocyte SREBP signaling mediates clock communication within the liver.
Guan, et al.
The Journal of Clinical Investigation, 133 (2023)
Mincle-GSDMD-mediated release of IL-1I? small extracellular vesicles from hepatic macrophages in ethanol-induced liver injury.
Zhang, et al.
Hepatology communications, 7 (2023)
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