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OP80

Sigma-Aldrich

Anti-APC (Ab-7) Mouse mAb (CC-1)

Synonym(s):

Anti-Adenomatus Polyposis Coli

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.45

biological source

mouse

Quality Level

100

clone

CC-1, monoclonal

form

liquid

contains

≤0.1% sodium azide

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (frozen sections): suitable

dilution

(Free-floating SectionsParaffin Sections)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

General description

Recognizes APC in oligodendrocytes and astrocytes.

  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
  • Mu Entrez ID: 11789
  • RatEntrez ID: 24205

Immunogen

a recombinant protein consisting of amino acids 1-226 of APC

Application

Frozen Sections (5 µg/ml) Immunofluorescence (5 µg/ml) Paraffin Sections (5 µg/ml, heat pre-treament required) Immunocytochemistry (see application references) Free Floating Sections (see application references)

Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration

Warning

Toxicity: Standard Handling

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative control: MOPC 21 cells

Positive control: Purkinje cells or cerebellum tissue

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Groden et al.
Cell, 66(3), 589-600 (1991-08-09)
DNA from 61 unrelated patients with adenomatous polyposis coli (APC) was examined for mutations in three genes (DP1, SRP19, and DP2.5) located within a 100 kb region deleted in two of the patients. The intron-exon boundary sequences were defined for
L K Su et al.
Science (New York, N.Y.), 262(5140), 1734-1737 (1993-12-10)
Mutations of APC appear to initiate sporadic and inherited forms of human colorectal cancer. Although these mutations have been well characterized, little is known about the function of the APC gene product. Two cellular proteins that associate with APC were
K W Kinzler et al.
Science (New York, N.Y.), 251(4999), 1366-1370 (1991-03-15)
Recent studies have suggested the existence of a tumor suppressor gene located at chromosome region 5q21. DNA probes from this region were used to study a panel of sporadic colorectal carcinomas. One of these probes, cosmid 5.71, detected a somatically
R V Bhat et al.
Glia, 17(2), 169-174 (1996-06-01)
Mutations of the adenomatous polyposis coli (APC) tumor suppressor gene have been linked to familial polyposis, an inherited predisposition to colon cancer, and a high percentage of sporadic colon adenomas. Although this gene is best known for its role in
The APC gene product in normal and tumor cells
Smith, K.J., et al.
Proceedings of the National Academy of Sciences, 90, 2846-2850 (1993)
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