MABF3113

Anti-NKp30/CD337 Antibody, clone F252

• Synonym(s): Activating natural killer receptor p30, CD337, NK-p30, Natural cytotoxicity triggering receptor 3, Natural killer cell p30-related protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: F252, monoclonal
• mol wt: calculated mol wt 21.59 kDa; observed mol wt ~N/A kDa
• purified by: affinity chromatography
• species reactivity: human
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable; inhibition assay: suitable; neutralization: suitable
• isotype: IgMκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001138938
• UniProt accession no.: O14931
• storage temp.: -10 to -25°C
• Gene Information: human ... NCR3(259197)

Description

Specificity

Clone F252 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30/CD337). It targets an epitope within the extracellular domain.

Immunogen

Activated human natural killer (NK) cells.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1 µg of this antibody detected NKp30 in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry application. (Meazza, R., et al. (2020). Cancers (Basel). 12(8):2187; Salome, B., et al. (2022). Cancer Cell. 40(9):1027-1043.e9).

Inhibition: A representative lot Inhibited the lysis of antigen presenting cells (APC) and blocked the expression of CD83, CD86.(Spaggiari, G.M., et al. (2001). Eur J Immunol.;31(6):1656-65; Vitale, M., et al. (2005). Blood. 106(2):566-71).

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Human peripheral blood mononuclear cells (PBMC). (Courtesy of Dr. Raffaella Meazza, IRCCS Ospedale Policlinico San Martino, Genova, Italy).

Neutralizing: A representative lot neutralized the receptor function of NKp30 (CD337). (Witkowski, M., et al. (2021). Nature. 600(7888):295-301).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Natural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). It is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of NK cells. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1 and stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF- and interferon- by NK cells that promote DC maturation. Masking of NKp30 with specific monoclonal antibodies is reported to reduce maturation of DCs cocultured with NK cells. Addition of supernatant from NK cells stimulated via NKp30 is shown to induce DC maturation. Six isoforms of NKp30 have been described that are produced by alternative splicing. (Ref.: Vitale, M., et al. (2005). Blood. 106(2); 566-571; Pende, D., et al. (1999). J. Exp. Med. 190(10); 1505-1516).

Physical form

Purified mouse monoclonal antibody IgM in PBS without preservatives.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable