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AP1160

Sigma-Aldrich

Anti-NDRG1 Mouse mAb (2D7)

liquid, clone 2D7, Calbiochem®

Synonym(s):

Anti-N-myc Downstream Regulator 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2D7, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NDRG1(10397)

General description

Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.
Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.
This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.

Immunogen

Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (0.7 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human endometrium tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Jiang, K., et al. 2010. Scand. J. Gastroenterol45, 898.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lianghui Dong et al.
PLoS pathogens, 17(6), e1009645-e1009645 (2021-06-03)
The presumed DNA helicase encoded by ORF44 of Kaposi's sarcoma-associated herpesvirus (KSHV) plays a crucial role in unwinding viral double-stranded DNA and initiating DNA replication during lytic reactivation. However, the regulatory mechanism of KSHV ORF44 has not been fully elucidated.

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