cGMP-Dependent Protein Kinase Catalytic Subunit, PKG Iα Catalytic Subunit, Protein Kinase G Iα, cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα
Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.
Physical form
In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95. Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589. Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391. Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
