accuracy: 80-120% (minimum of 10 samples spiked with acceptable recovery)
technique(s)
ELISA: suitable single molecule counting: suitable
General description
Interleukin-10 (IL-10) is a cytokine with both stimulatory and suppressive effects on the immune system. The protein originates from several cell lines, including Th2 cells, mast cells, macrophages, monocytes and B-cells. Its main functions are to impede inflammatory responses and to inhibit synthesis of other pro-inflammatory cytokines, such as IL-2, IL-3, GM-CSF, IFN-γ and TNF-α. Besides its anti-inflammatory capabilities, IL-10 also regulates the production of natural killer cells and lymphocytes. Due to its multiple roles, proper function of IL-10 is required to maintain a healthy immune system. Improper function of IL-10 can result in disease development, as over expression of IL-10 has been linked to lymphoma, whereas IL-10 deficiency has implications for Crohn′s disease.
The SMC® Mouse IL-10 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Application
Research Category Inflammation & Immunology
Research Sub Category Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Mouse IL-10 Discovery Immunoassay has unparalleled performance in quantifying IL-10 in mouse serum.
Used to detect/quantify:IL-10
Components
Assay Buffer Mouse IL-10 Coating Antibody Assay Plate 10X PBS Blocking Buffer Standard Diluent Mouse IL-10 Detection Antibody Mouse IL-10 Standard SMC® Mouse IL-10 Immunoassay Kit Instructions 10X Wash Buffer Buffer C Elution Buffer B
Analysis Note
Low-end CV% Range: 0-11% Low-end CV% Average: 6%
Legal Information
SMC is a registered trademark of Singulex
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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