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SRP6480

Sigma-Aldrich

Fas/CD95 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

Synonym(s):

ALPS1A, APO-1, APO1, APT-1, APT1, CD-95, CD95, FAS-1, FAS1, FASTM, Fas-R, FasR, TNFRSF-6, TNFRSF6

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥92% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 18.2 kDa
observed mol wt 25-35 kDa by SDS-PAGE (DTT-reduced. Gln 26 is the predicted N-terminal.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FAS(355)

General description

Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). The FASR gene is mapped to chromosome 10 and 19 in humans and mice respectively.

Biochem/physiol Actions

The FAS (Fas cell surface death receptor) gene encodes a death receptor on the surface of cells that leads to programmed cell death (apoptosis). Cross linking between the Fas receptor (FasR) and ligand triggers apoptotic-signaling. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Fas mediated apoptosis inhibits autoreactive B and T cells which is misunderstood to maintain immune homeostasis. Thus, Fas ligand (FasL) associated apoptosis offers protection against autoimmune diseases such as. Mutation in the FAS gene leads to the risk of autoimmune disorder including Guillain-Barré syndrome, systemic lupus erythematosus (SLE), multiple sclerosis, primary Sjogren′s syndrome Trimerization of the FasR is initiated when the membrane anchored FasL trimers along the surface of adjacent cells. A similar event is induced by agonistic Fas antibody. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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FAS promoter polymorphisms and serum sFas level are associated with increased risk of nerve damage in Bangladeshi patients with Guillain-Barre syndrome.
Islam Z, et al.
PLoS ONE, 13(2), e0192703-e0192703 (2018)
Resveratrol and cellular mechanisms of cancer prevention.
Shukla Y and Singh R
Annals of the New York Academy of Sciences, 1215(1), 1-8 (2011)
FLICE-inhibitory proteins: regulators of death receptor-mediated apoptosis.
Krueger A, et al.
Molecular and Cellular Biology, 21(24), 8247-8254 (2001)
Genetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis.
Mou H, et al.
Proceedings of the National Academy of Sciences of the USA, 201620861 (2017)

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