SRP6103

MDH1 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): MDH-s, MDHA, MOR2, Malate dehydrogenase cytoplasmic
• CAS Number: 480083-12-3
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 37.4 kDa (342 aa, 1-334 aa + CT His Tag)
• packaging: pkg of 100 μg
• technique(s): activity assay: suitable
• NCBI accession no.: P40925
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MDH1(4190)

Description

General description

MDH1 (malate dehydrogenase 1) is cytoplasmic protein belonging to the 2-hydroxy acid dehydrogenases protein family. Eukaryotes contain two forms of MDH proteins depending upon their subcellular localization, MDH1 and MDH2. MDH2 is present in the mitochondrial matrix and MDH1 is present in the cytoplasm. This gene is localized to human chromosome 2, and is composed of nine exons and eight introns.

Application

MDH1 (malate dehydrogenase 1) human has been used for measuring MDH1 enzyme activity.

Biochem/physiol Actions

MDH1 (malate dehydrogenase 1) is responsible for reversibly converting malate to oxaloacetate in cytoplasm. It participates in metabolism where it transports NADH equivalents and metabolites across the mitochondrial membrane, and regulates the TCA (tricarboxylic acid) cycle pool size. MDH1 is involved in malate/aspartate shuttle, which is involved in maintaining the balance of nitrogen, oxaloacetate, and the α-ketoglutarate intermediate shuttling between the cytosol and mitochondrial matrix. It is thought to function as an important checkpoint for energy balance between mitochondria and cytoplasm. MDH1 acts as a regulator of p53-dependent apoptosis in response to glucose deprivation, indicating that synergism between energy metabolism and p53 transactivation is linked with cell metabolic state and hence, determination of cell death.

Physical form

1 mg/mL solution in 20 mM Tris-HCl (pH 8.0) containing 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable