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F3378

Sigma-Aldrich

Fast Blue BB Salt hemi(zinc chloride) salt

Dye content ≥80 %, Powder

Synonym(s):

4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Linear Formula:
C17H18N3O3Cl · 1/2 ZnCl2
CAS Number:
Molecular Weight:
415.94
Colour Index Number:
37175
Beilstein:
1845288
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Fast Blue BB Salt hemi(zinc chloride) salt, Dye content ≥80 %

form

powder

composition

Dye content, ≥80%

mp

157 °C

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4

InChI

1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2

InChI key

CMFRFQODFZBKTI-UHFFFAOYSA-L

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General description

Fast Blue BB Salt hemi(zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt.

Application

Fast Blue BB Salt hemi(zinc chloride) salt has been used for alkaline phosphatase staining in cells.
Fast blue BB salt is suitable for the staining of enterochromaffin. Its applications are seen in enzyme histochemistry by Gomori and are suitable for the histochemical demonstration of beta-glucuronidase activities, and various peptidases and proteases. It is also used in enzyme histochemistry including the demonstration of serine esterase in blood smears and histological sections. Fast blue BB salt plays a vital role as a routine stain to demonstrate alkaline phosphatase labeled antibodies.

Preparation Note

Purified

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Peripheral deletion of autoreactive CD8+ T cells in transgenic mice expressing H-2Kb in the liver.
Bertolino P
European Journal of Immunology, 25, 1932-1932 (1995)
I Ojanperä et al.
Journal of analytical toxicology, 12(2), 108-110 (1988-03-01)
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We are reporting what we believe to be a safe, rapid TLC system and spray for the identification of marihuana. The use of Fast Blue 2B salt greatly enhances the specificity of the TLC analysis of suspected marihuana samples.

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