C0730
Anti-CD137 antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-4-1BB
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About This Item
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biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
capture ELISA: 0.2-0.8 μg/mL
flow cytometry: 2.5 μg using 106 human T cells treated with PHA
immunocytochemistry: 5-15 μg/mL
western blot: 0.1 μg/mL
impurities
<0.10 EU/μg endotoxin
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TNFRSF9(3604)
General description
CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.
Immunogen
mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu24Gln186
Application
Anti-CD137 antibody produced in goat is suitable for the following applications:
- Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
- ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
- Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
- Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
- Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.
Biochem/physiol Actions
CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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G Kienzle et al.
International immunology, 12(1), 73-82 (1999-12-23)
Human CD137 is a member of the tumor necrosis factor (TNF) receptor family and the homologue of murine 4-1BB. Recent studies have demonstrated that CD137 promotes accessory T cell activation, and regulates proliferation and survival of T lymphocytes. This study
J Langstein et al.
Journal of leukocyte biology, 65(6), 829-833 (1999-06-25)
CD137 (ILA/4-1BB), a member of the tumor necrosis factor (TNF) receptor family, promotes adherence and prolongs survival of human peripheral monocytes. It induces a strong expression of macrophage colony-stimulating factor (M-CSF), an essential monocyte survival factor. Monocyte survival induced by
J Langstein et al.
Journal of immunology (Baltimore, Md. : 1950), 160(5), 2488-2494 (1998-03-14)
CD137 (ILA/4-1BB), a member of the TNF receptor family, was shown previously to inhibit proliferation and to induce apoptosis in T lymphocytes. In this study, we identify CD137 as a novel and potent monocyte activation factor. CD137 protein induces expression
H H Kim et al.
Molecules and cells, 10(3), 247-252 (2000-07-20)
It has been widely accepted that T cell activation requires two signals; one from the binding of the antigen/major histocompatibility complex to the T-cell receptor (TCR)/CD3 complex and the other from the interaction between a surface molecule on antigen presenting
J C Hurtado et al.
Journal of immunology (Baltimore, Md. : 1950), 155(7), 3360-3367 (1995-10-01)
The expression of the murine T cell Ag 4-1BB, a member of the TNF-R family, is induced by T cell activation. Previously, we and others had shown that signaling through 4-1BB enhanced proliferative T cell responses. To investigate a potential
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