AL11430
DMT-locA(bz) Phosphoramidite
configured for ABI
About This Item
Recommended Products
biological source
non-animal source (BSE/TSE no Risk)
Quality Level
product line
Proligo Reagents
Assay
≥98.0% (31P-NMR)
≥98.0% (reversed phase HPLC)
form
powder
impurities
≤3 wt. % Residual Solvent Content
<0.4% Water Content (Karl Fischer)
<0.5% Single unspecified Impurity (reversed phase HPLC)
color
white to off-white
solubility
soluble, clear, colorless
absorption
<0.1 in acetonitrile at 0.2 M
suitability
conforms to structure for H-NMR
conforms to structure for LC-MS
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | CL11430 | A111000 | C111000 |
---|---|---|---|
300 | 300 | 200 | 200 |
powder | powder | powder or granules | powder or granules |
≥98.0% (31P-NMR), ≥98.0% (reversed phase HPLC) | ≥98.0% (31P-NMR), ≥98.0% (reversed phase HPLC) | ≥99% (31P-NMR), ≥99.0% (reversed phase HPLC) | ≥99% (31P-NMR), ≥99.0% (reversed phase HPLC) |
white to off-white | white to off-white | white to off-white | white to off-white |
−20°C | −20°C | 2-8°C | 2-8°C |
General description
With the exception of the LNA monomers, LNA synthesis is accomplished with the same reagents as DNA synthesis. LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran (THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.
Features and Benefits
- LNA oligonucleotides are prepared by phosphoramidite chemistry
- Standard DNA synthesizer platforms can be employed. No change is required in the reagents commonly used for DNA synthesis
- To further enhance the hybridization characteristics of LNA, 5-methyl-cytidine is employed instead of cytidine
- LNA monomers are as soluble in acetonitrile as their DNA counterparts (except for the 5-methyl-cytidine derivative, which requires the application of 10-20%, dichloromethane as a co-solvent)
- Mixmer oligonucleotides containing LNA, DNA and/or RNA monomers can be assembled easily
- LNA oligonucleotides with predefined melting temperatures (Tm) can be designed and prepared
Other Notes
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Need A Sample COA?
This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service