86186

Sulforhodamine B acid chloride

suitable for fluorescence, technical

• Synonym(s): Lissamine^™ rhodamine B sulfonylchloride
• Empirical Formula (Hill Notation): C₂₇H₂₉ClN₂O₆S₂
• CAS Number: 62796-29-6
• Molecular Weight: 577.11
• Beilstein: 7734927
• EC Number: 263-735-8
• MDL number: MFCD00042001
• UNSPSC Code: 12352106
• PubChem Substance ID: 57653085
• NACRES: NA.32

Properties

• grade: technical
• form: powder
• fluorescence: λ_ex 560 nm; λ_em 580 nm in chloroform; λ_ex 560 nm; λ_em ~580 nm in chloroform
• suitability: suitable for fluorescence
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: CCN(CC)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(Cl)(=O)=O)=[N+](/CC)CC)c1
• InChI: 1S/C27H29ClN2O6S2/c1-5-29(6-2)18-9-12-21-24(15-18)36-25-16-19(30(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(28,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3
• InChI key: YERWMQJEYUIJBO-UHFFFAOYSA-N

Description

General description

Sulforhodamine B is a fluorescent dye.

Application

Sulforhodamine B acid chloride has been used to synthesize rhodamine-labeled polycation (PLL-ROD). It has also been used in fluorescent protein tracer preparation.

Fluorescent protein label forming stable conjugates

Biochem/physiol Actions

The sulforhodamine B (SRB) assay is useful in determining the density of cells.

Legal Information

Lissamine is a trademark of PerkinElmer Inc.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302,H315,H319,H335
• Precautionary Statements: P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves