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T1258

Sigma-Aldrich

Trizma® acetate

Trizma® acetate

≥99.0% (titration)

Synonym(s):

TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

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About This Item

Linear Formula:
NH2C(CH2OH)3 · CH3COOH
CAS Number:
6850-28-8
Molecular Weight:
181.19
Beilstein:
3702918
EC Number:
229-939-6
MDL number:
MFCD00038951
UNSPSC Code:
12161700
PubChem Substance ID:
24899948
NACRES:
NA.25
Pricing and availability is not currently available.

Assay

≥99.0% (titration)

form

crystalline powder

pH

6.0-7.0 (0.1 M in water, high purity)

solubility

water: 0.5 M, clear, colorless

SMILES string

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

PIEPQKCYPFFYMG-UHFFFAOYSA-N

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Application

Trizma® acetate has been used:
  • in the adenosine triphosphate (ATP) assay to neutralize the pH to 7.7 of the oocyte supernatant[1]
  • in cell wall isolation to remove proteins and membranes from the cell walls of plants[2]
  • as a component of DamID buffer, lysis buffer for cell sorting and lysing[3]

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000435951
0000435951
0000424197
0000424197
0000424211

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S Hashimoto et al.
Molecular reproduction and development, 57(4), 353-360 (2000-11-07)
The effects of carbohydrates on meiotic maturation and ATP content of bovine oocytes under low oxygen tension (5%) were investigated. Furthermore, the developmental competence or intracellular H(2)O(2) contents of the oocytes matured under 5% or 20% O(2) was assessed. In
Máté Borsos et al.
Nature, 569(7758), 729-733 (2019-05-24)
In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here we generate high-resolution maps of genomic
Máté Borsos et al.
Nature, 569(7758), 729-733 (2019-05-24)
In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here we generate high-resolution maps of genomic
Masanobu Yamamoto et al.
ChemSusChem, 13(17), 4400-4408 (2020-07-22)
Increases in coniferaldehyde content, a minor lignin residue, significantly improves the sustainable use of plant biomass for feed, pulping, and biorefinery without affecting plant growth and yields. Herein, different analytical methods are compared and validated to distinguish coniferaldehyde from other
1 Review This action will navigate to reviews.

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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