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S4320

Sigma-Aldrich

SYBR® Green Extract-N-Amp™ PCR ReadyMix™

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

Synonym(s):

SYBR Green PCR Mix

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About This Item

UNSPSC Code:
41106303
NACRES:
NA.31

Quality Level

200

usage

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
 sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
 sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

feature

dNTPs included
hotstart

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

The SYBR® Green Extract-N-Amp™ PCR ReadyMix is the specially formulated PCR enzyme blend contained in the SYBR Green Extract-N-Amp Plant PCR kit and SYBR Green Extract-N-Amp Tissue PCR Kit. It is intended to supplement these kits for researchers who perform more than one amplification per extracted sample.

Application

SYBR® Green Extract-N-Amp™ PCR ReadyMix™ or SyBr Green 2× Master Mix kit has been used in a real-time quantitative polymerase chain reaction (qRT–PCR).

Packaging

1.2 mL is sufficient for 100 amplifications as described in kit protocols (20 μl total reaction volume)

12 mL is sufficient for 1000 amplifications as described in kit protocols (20 μl total reaction volume)

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

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XNAP

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAPE

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications

XNAP2

Extract-N-Amp™ Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAR

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XNAPR

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAPS

REDExtract-N-Amp™ Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

XNAT2

Extract-N-Amp™ Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

XNAT2R

Extract-N-Amp™ Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications

XNAT

REDExtract-N-Amp™ Tissue PCR Kit, sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

R4775

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E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

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SYBR® Green Extract-N-Amp™ Plant PCR Kit, sufficient for 100 preparations

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SYBR® Green Extract-N-Amp™ Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications

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E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
Ririe KM, Rasmussen RP, Wittwer CT
Analytical Biochemistry, Feb, 154-160 (1997)
Vikas Yadav Patade et al.
Protoplasma, 248(3), 613-621 (2010-09-21)
Identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. In this study, PCR-based cDNA suppression subtractive hybridization technique was used to construct sugarcane salt (NaCl) stress
Sudhakar Srivastava et al.
Journal of experimental botany, 64(1), 303-315 (2012-11-20)
MicroRNAs (miRNAs) constitute a novel mechanism of gene regulation affecting plant development, growth, and stress response. To study the role of miRNAs in arsenic (As) stress, microarray profiling of miRNAs was performed in Brassica juncea using a custom Phalanx Plant
Karuna Yadav et al.
PloS one, 12(11), e0188933-e0188933 (2017-12-01)
Iron is an indispensable element for plant growth and defense and hence it is essential to improve the plant's ability to accumulate iron. Besides, it is also an important aspect for human health. In view of this, we attempted to
Archana N Rai et al.
3 Biotech, 10(3), 114-114 (2020-03-03)
Heat stress is one of the major limitations to crop productivity. In the present study, an efficient method of screening was adopted for identification of heat tolerant Indian Mustard genotypes by applying 4-day cycle of heat stress to seedlings. Thirty-four genotypes

Articles

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Protocols

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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