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RAB0252

Sigma-Aldrich

Human IL-12 p70 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type urine
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.8-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL12A(3592)

Related Categories

General description

The Human IL-12(P70) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12(P70) in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human IL-12 p70

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IL-12 p70 ELISA kit has been used to determine the concentrations of IL-12, IL-10, IL-1β and TNFα in cell culture supernatants.

Biochem/physiol Actions

IL-12A (interleukin 12 A) is essential for differentiation of Th1 (type 1) cells. Polymorphisms in IL-12A gene is associated with Graves′ and Alzheimer′s disease, rheumatoid arthritis and asthma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interleukin 35 polymorphisms are associated with decreased risk of premature coronary artery disease, metabolic parameters, and IL-35 levels: the genetics of atherosclerotic disease (GEA) study.
Posadas-Sanchez R, et al.
Mediators of Inflammation, 2017, 10-10 (2017)
Brucella Omp25 Upregulates miR-155, miR-21-5p, and miR-23b to Inhibit Interleukin-12 Production via Modulation of Programmed Death-1 Signaling in Human Monocyte/Macrophages.
Cui B, et al.
Frontiers in Immunology, 8, 708-708 (2017)
Jianfei Ji et al.
Stem cells translational medicine, 3(3), 356-364 (2014-01-01)
Targeting cancer stem cells (CSCs) with immunotherapy may be an effective means to prevent recurrences in glioblastoma multiforme (GBM). It is well established that CD133 is expressed in the population of GBM tumor cells representing CSCs. This raises a possibility
Xiaolu Sun et al.
BMC molecular and cell biology, 23(1), 14-14 (2022-03-21)
Pulmonary tuberculosis (TB) is a chronic infectious disease. microRNA (miR)-378 is involved in TB diagnosis. This study explored the effects of miR-378 on TB patients. A total of 126 TB patients were selected, including 63 active TB and 63 latent TB
D M Bailey et al.
Acta physiologica (Oxford, England), 212(2), 175-187 (2014-05-09)
The aim of this study was to examine if erythropoietin (EPO) has the potential to act as a biological antioxidant and determine the underlying mechanisms. The rate at which its recombinant form (rHuEPO) reacts with hydroxyl (HO˙), 2,2-diphenyl-1-picrylhydrazyl (DPPH˙) and
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