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P4109

Sigma-Aldrich

β-Phosphoglucomutase from Lactococcus sp.

recombinant, expressed in E. coli

Synonym(s):

β-D-Glucose 1,6-phosphomutase

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

solid

specific activity

≥10 unit/mg solid

storage temp.

−20°C

Application

β-Phosphoglucomutase is used to study starch and sucrose metabolism. It is used for the determination of α-amylase in clinical analysis. It is used to study galactose utilization and generalized glycogenosis (Pompe′s Disease).

Biochem/physiol Actions

β-Phosphoglucomutase enzymatically converts β-D-glucose 1-phosphate to β-D-glucose 6-phosphate. It is involved in starch and sucrose metabolism.
Enzymatically converts β-D-glucose-1-phosphate to β-D-glucose-6-phosphate.

Unit Definition

One unit is defined as the amount of enzyme which converts 1μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per minute at 37°C and pH 7.0.

Other Notes

Contains EDTA and lactose.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Brigitta Elsässer et al.
Journal of molecular modeling, 18(7), 3169-3179 (2012-01-13)
Enzyme catalyzed phosphate transfer is a part of almost all metabolic processes. Such reactions are of central importance for the energy balance in all organisms and play important roles in cellular control at all levels. Mutases transfer a phosphoryl group
F Levander et al.
Applied and environmental microbiology, 67(6), 2734-2738 (2001-05-26)
To study the influence of phosphoglucomutase (PGM) activity on exopolysaccharide (EPS) synthesis in glucose- and lactose-growing Streptococcus thermophilus, a knockout PGM mutant and a strain with elevated PGM activity were constructed. The pgmA gene, encoding PGM in S. thermophilus LY03
Cecelia A Garcia et al.
Applied microbiology and biotechnology, 105(10), 4033-4052 (2021-05-08)
In a competitive microbial environment, nutrient acquisition is a major contributor to the survival of any individual bacterial species, and the ability to access uncommon energy sources can provide a fitness advantage. One set of soluble carbohydrates that have attracted
A phosphoglucomutase specific for β-glucose 1-phosphate
Ben-Zvi R and Schramm M
The Journal of Biological Chemistry, 236, 2186-2189 (1961)
OCULAR HISTOPATHOLOGY IN GENERALIZED GLYCOGENOSIS (POMEPE'S DISEASE).
D TOUSSAINT et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 73, 342-349 (1965-03-01)

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