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MAK311

Sigma-Aldrich

Peroxide Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Hydrogen Peroxide Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders

storage temp.

2-8°C

Related Categories

General description

Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.[1]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.

Principle

The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ −xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
311CE12A16
311CE07A01
311CD11A17
311CD05A23
311CD05A24

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Steffen Pabel et al.
Circulation research, 130(7), 994-1010 (2022-02-24)
Atrial fibrillation (AF) and heart failure often coexist, but their interaction is poorly understood. Clinical data indicate that the arrhythmic component of AF may contribute to left ventricular (LV) dysfunction. This study investigates the effects and molecular mechanisms of AF
Dhiman Chakravarty et al.
Bioresource technology, 293, 122013-122013 (2019-09-09)
Here, we show the utility of a cyanobacterial biomass for overproduction and easy downstream processing of the thermostable protein KatB (a Mn-catalase). The nitrogen-fixing blue-green alga, Anabaena, was bioengineered to overexpress the KatB protein (An-KatB). Interestingly, pure An-KatB could be
Role of Cystathionine Gamma Lyase in Diabetic Nephropathy.
Anter A F, et al.
Journal of Advanced Biomedical and Pharmaceutical Sciences, 1(1 ), 33-40 (2018)
T O Ajiboye et al.
International journal of antimicrobial agents, 53(4), 467-473 (2018-12-12)
Zinc is a highly coveted redox-inactive micronutrient required for the growth and virulence of Acinetobacter baumannii. In this study, the role of the zinc uptake regulator Zur in the susceptibility and oxidative stress response of A. baumannii to antibiotics was
Han Xie et al.
Redox biology, 53, 102340-102340 (2022-05-25)
Generation of reactive oxygen species (ROS) are possibly induced by the crosstalk between mitochondria and endoplasmic reticula, which is physiologically important in apoptosis. Cytochrome c (Cyt c) is believed to play a crucial role in such signaling pathway by interrupting
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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is it possible to use the MAK311-1KT Peroxide Assay Kit to measure peroxide in lysed cells that are grown in Minimum Essential Medium Eagle containing Phenol Red at a concentration of 0.011 g/liter?

    1 answer

    1. The reagents used with the MAK311-1KT Peroxide Assay Kit create an acidic environment in the well plate, and Phenol Red should not have measurable absorbance at the kit's wavelength of 585 nm.
      As an extra precaution, it is recommended to dilute the H2O2 standard in the same media that is used on the cells to account for any background or chemical interactions from the media. Furthermore, it is best for samples to contain less than 1 mM pyruvate.

      Helpful?

  4. Is 1% TWEEN in the sample compatible with the MAK311 peroxide assay kit?

    1 answer

    1. The tween in samples should remain below 0.25%. If the sample contains 1% tween it is recommend diluting 4x to 0.25%, assuming the sample has enough peroxide to still be in the detectable range. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.

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