I2393

Interleukin-1β from rat

IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-1β, Interleukin-1β from rat, Lymphocyte activating factor
• MDL number: MFCD01094098
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: rat
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• potency: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• mol wt: 17.3 kDa
• packaging: pkg of 10 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg
• UniProt accession no.: Q63264
• storage temp.: −20°C
• Gene Information: rat ... Il1b(24494)

Description

General description

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.¹ IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,² and is thought to play a key role in inflammatory and immune responses.³ Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRF_H), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).⁴ The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured in a cell proliferation assay using murine D10S cells.

Citation

1. Gery, I., et al., J. Exp. Med., 136, 128 (1972).
2. Oppenheim, J. J., et al., Immunol. Today, 7, 45 (1986).
3. Durum, S. K., et al., Annu. Rev. Immunol., 3, 263 (1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
5. Symons, J., et al., Lymphokines and Interferons, A Practical Approach, Clemens, M., et al., (eds.), IRL Press, Oxford (1987).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves