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F9880

Sigma-Aldrich

Fluorinert™ FC-70

Synonym(s):

Perfluorotriamylamine, fluorocarbon FC 70

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About This Item

CAS Number:
338-84-1
EC Number:
206-421-8
MDL number:
MFCD00042367
UNSPSC Code:
15121530
PubChem Substance ID:
24895003
NACRES:
NA.28
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description

used in density gradient studies

Quality Level

100

density

1.93 g/mL at 25 °C (lit.)

SMILES string

FC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)N(C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F

InChI

1S/C15F33N/c16-1(17,4(22,23)10(34,35)36)7(28,29)13(43,44)49(14(45,46)8(30,31)2(18,19)5(24,25)11(37,38)39)15(47,48)9(32,33)3(20,21)6(26,27)12(40,41)42

InChI key

AQZYBQIAUSKCCS-UHFFFAOYSA-N

Application

Fluorinert™ FC-70 has been used to reduce the adherence of large trypsin:N-amidinopiperidine crystals to the well.[1] It has also been used as a fluorocarbon oil for microinjection of oocytes.[2][3]
For use as a high density chase solution in density gradient studies.

Packaging

Packaged and distributed by Sigma-Aldrich

Other Notes

Fluorinert FC-70 is a liquid mixture of completely fluorinated aliphatic compounds.

Legal Information

Fluorinert is a trademark of 3M Company

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCS8653
MKCS0686
MKCS4578
MKCQ6517
MKCN2223

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Quantitative microinjection of oocytes, eggs, and embryos
Jaffe LA and Terasaki M
Methods in Cell Biology, 74, 219-242 (2004)
Alexandra Colin et al.
The Journal of cell biology, 219(3) (2020-01-18)
Nucleus centering in mouse oocytes results from a gradient of actin-positive vesicle activity and is essential for developmental success. Here, we analyze 3D model simulations to demonstrate how a gradient in the persistence of actin-positive vesicles can center objects of
Microinjection: Methods and Applications (2016)
Intriguing role of water in protein-ligand binding studied by neutron crystallography on trypsin complexes
Schiebel J, et al.
Nature Communications, 9 (2018)

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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