C8093

Anti-Connexin 43 Antibody

mouse monoclonal, CXN-6

• Synonym(s): Mouse monoclonal antibody
• MDL number: MFCD00803532
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• product name: Monoclonal Anti-Connexin-43 antibody produced in mouse, clone CXN-6, ascites fluid
• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: CXN-6, monoclonal
• mol wt: antigen 43 kDa
• contains: 15 mM sodium azide
• species reactivity: rat, hamster, bovine, mouse, human
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1:8,000-1:16.000 using mouse whole brain extract
• isotype: IgM
• UniProt accession no.: P17302
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GJA1(2697) , GJA1P1(2698) ; mouse ... Gja1(14609) ; rat ... Gja1(24392)

Description

General description

Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.

The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes.

Specificity

Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.

Immunogen

synthetic connexin-43 peptide (362-381).

Application

Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in:

• fluorescent immunostaining
• western blotting
• immunocytochemistry
• preparation of immuno-affinity columns

Biochem/physiol Actions

Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable