A8549

Albumin, biotin labeled bovine

lyophilized powder

• Synonym(s): Biotin labeled albumin
• CAS Number: 9048-46-8
• MDL number: MFCD00130395
• UNSPSC Code: 12352202
• eCl@ss: 34058011
• NACRES: NA.25

Properties

• biological source: bovine
• Quality Level: 200
• Assay: ≥80% protein basis (biuret)
• form: lyophilized powder
• concentration: ≥97% protein in anhydrous DMSO (spectralphotometric; calc. on anhydrous substance); ≥98% (Assay of Albumin (Agarose gel electrophoresis))
• extent of labeling: 8-16 mol biotin per mol albumin
• technique(s): bioconjugation: suitable
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

Application

Biotin labeled bovine albumin has been used as a component in myosin V processivity experiments.[1] It has also been used to provide biotin tags for neutravidin coating and also to suppress nonspecific cell adhesion during preparation of tension gauge tether (TGT) surface.[2]

Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues.

Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues. In spleen, biotin-BSA binds to red pulp sinuses, but not white pulp vessels (including the central arteriole) nor the marginal sinus. In the thymus, prominent binding was restricted to the capillaries of the corticomedullary area.

Physical form

Contains sodium citrate buffer salts

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves