Skip to Content
Merck
All Photos(5)

Key Documents

View All Documentation

A5503

Sigma-Aldrich

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym(s):

Albumin from hen egg white, Ovalbumin

Sign Into View Organizational & Contract Pricing
All Photos(5)

About This Item

CAS Number:
9006-59-1
EC Number:
232-692-7
MDL number:
MFCD00130424
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25
Pricing and availability is not currently available.

biological source

chicken egg white

Quality Level

300

type

Grade V

Assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

crystallization

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 40 mg/mL

UniProt accession no.

P01012

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Chicken egg albumin, or ovalbumin, is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated glycoprotein. From the amino acid sequence, the peptide portion of ovalbumin consists of 385 residues and has a molecular weight of 42.7 kDa.[1]

Application

A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.
Ovalbumin is useful as a marker (∼45 kDa) for protein electrophoresis. Ovalbumin has been used to produce an animal model of asthma. Ovalbumin was used as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophils.[2]
Studies to test the effects of localization on DNA immunizations use ovalbumin in antibody assays.[3]
Ovalbumin has been used as an induction agent in model studies of atopic dermatitis.

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C.[4] Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.

Physical form

Essentially salt free

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1A

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000417379
0000417380
0000417381
0000384106
0000384106

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J S Boyle et al.
International immunology, 9(12), 1897-1906 (1998-02-18)
We examined the role of the cellular localization of antigen on the immune response after DNA immunization of mice with three forms of ovalbumin (OVA). DNA encoding OVA which was secreted (sOVA) generated 10- to 100-fold higher IgG responses with
A D Nisbet et al.
European journal of biochemistry, 115(2), 335-345 (1981-04-01)
The complete amino acid sequence of hen ovalbumin, comprising 385 residues, has been determined. The sequence was deduced from the 17 cyanogen bromide fragments and from peptides derived by digestion with a number of proteolytic enzymes. The molecular weight of
Joseph Khoory et al.
PloS one, 11(1), e0141206-e0141206 (2016-01-20)
Acute, inflammatory conditions associated with dysregulated complement activation are characterized by significant increases in blood concentration of reactive oxygen species (ROS) and ATP. The mechanisms by which these molecules arise are not fully understood. In this study, using luminometric- and
Christianne Bandeira-Melo et al.
Journal of immunological methods, 276(1-2), 227-237 (2003-05-10)
Eosinophils contain several preformed cytokines within their specific granules. Therefore, without requiring them in de novo synthesis of cytokines, eosinophils can release quantities of granule-derived cytokines by highly regulated mechanisms. However, eosinophil "degranulation" is poorly understood, in part, because available
A differential scanning calorimetric study of conversion of ovalbumin to S-ovalbumin in eggs.
J W Donovan et al.
Journal of the science of food and agriculture, 27(2), 197-204 (1976-02-01)
View All Related Papers

Articles

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Questions

1–9 of 9 Questions  

  1. Is this endotoxin free ovalbumin?

    1 answer

    1. Unfortunately, this product is not tested for endotoxin.

      Helpful?

  2. Is A5503 fluorescent?

    1 answer

    1. This product is pure ovalbumin with a minimum purity specification of 98%. No fluorescent conjugate or probe is present in this material.

      Helpful?

  3. Does A5503 can analyze by western blotting?

    1 answer

    1. This product has not been specifically tested for use in western blot. However, there is no reason to suspect that this albumin would not be suitable.

      Helpful?

  4. What is the solubility of Product No. A5503, Albumin from chicken egg white, in aqueous buffers (e.g. Tris, PBS)?

    1 answer

    1. We have only tested solubility in water. Salts in buffers may make solubility more difficult.

      Helpful?

  5. How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?

    1 answer

    1. A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE.

      Helpful?

  6. Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?

    1 answer

    1. We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.

      Helpful?

  7. Product No. A5503, Albumin from chicken egg white, is to be stored at 2 - 8°C. Does it ship on ice?

    1 answer

    1. A5503 does not require shipping on ice.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?

    1 answer

    1. A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service