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53608

Sigma-Aldrich

Biotin (5-fluorescein) conjugate

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide

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About This Item

Empirical Formula (Hill Notation):
C33H32N4O8S
CAS Number:
957494-27-8
Molecular Weight:
644.69
MDL number:
MFCD03791084
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
57651424
NACRES:
NA.32
Pricing and availability is not currently available.

product line

BioReagent

Quality Level

100

Assay

≥90% (HPCE)

solubility

DMSO: soluble

fluorescence

λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCNC(=O)c3ccc(c(c3)C(O)=O)C4=C5C=CC(=O)C=C5Oc6cc(O)ccc46)[C@@]1([H])NC(=O)N2

InChI

1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

InChI key

DFUFXKZUEOKPSD-LFERIPGTSA-N

Application

Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Biotin-fluorescein conjugate for the fluorometric, accurate measurement of avidin and streptavidin[1].
Often referred to as Biotin (4-fluorescein) conjugate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5272
BCCJ3383
BCCF8401
BCCB9180
BCCB2419

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High-resolution fiber-optic microendoscopy for in situ cellular imaging.
Pierce M, Yu D, Richards-Kortum R.
Journal of Visualized Experiments, 11, 2306-2306 (2011)
Ann L Baldwin et al.
Journal of the American Association for Laboratory Animal Science : JAALAS, 46(1), 58-65 (2007-01-06)
Housing rats in an environment with high personnel activity increases microvascular leakiness to albumin in the mesenteric microcirculation and causes mast cell degranulation. In this study, rats were exposed to daily 15-min episodes of 90-dB SPL noise to determine whether
Damien D Pearse et al.
The European journal of neuroscience, 20(12), 3387-3396 (2004-12-22)
Microglia respond in a stereotypical pattern to a diverse array of pathological states. These changes are coupled to morphological and immunophenotypical alterations and the release of a variety of reactive species, trophic factors and cytokines that modify both microglia and
H J Gruber et al.
Biochimica et biophysica acta, 1381(2), 203-212 (1998-08-01)
A new fluorimetric assay is presented for the specific and reliable quantitation of >/=2 nM avidin and streptavidin. The assay is based on pronounced changes in the fluorescence properties of commercial fluorescein-biotin, or of a newly synthesized biotin-poly(ethylene glycol)-pyrene conjugate
G. Kada et al.
Biochimica et Biophysica Acta, 1427, 33-33 (1998)

Questions

1–2 of 2 Questions  

  1. What is the extinction coefficient for product 53608.

    1 answer

    1. Although the excitation and emission wavelengths are measured during quality control testing, there is no testing to determine the extinction coefficient for 53608 (Biotin (5-fluorescein) conjugate). There was no information on file regarding the extinction coefficient for product 53608.

      Helpful?

  2. what are the solvents required to dilute these two reagents and also the recommended concentration?

    1 answer

    1. The solubility of Biotin (5-fluorescein) conjugate 53608-1MG-F is not a specification for product release, but it should be soluble in DMSO at least up to a concentration of 3 mM.

      Helpful?

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