42395

Tannase from Aspergillus ficuum

powder, white, ≥150 U/g

• Synonym(s): Tannin acyl Hydrolase
• CAS Number: 9025-71-2
• EC Number: 232-804-4
• MDL number: MFCD00212735
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Aspergillus sp. (A. ficuum)
• Quality Level: 200
• form: powder
• specific activity: ≥150 U/g
• impurities: 25 mM potassium phosphate; 250 mM NaCl; 50% glycerol
• color: white
• storage temp.: 2-8°C

Description

General description

Tannase is an enzyme that is produced by several organisms such as plants, bacteria, fungi, and yeast. This enzyme is also found in plant sources such as tannin-rich vegetables, especially in the leaves, fruits, branches, and bark.

Application

Tannase from Aspergillus ficuum has been used:

• as a standard to determine the tannase activity of bacterial isolates
• to study its effects on the inhibitory activity of tannic acid on biofilm formation
• to obtain Proanthocyanidins (PA) by enzymatic hydrolysis of grape skin and seeds

Biochem/physiol Actions

Tannase catalyzes the ester bonds located in complex tannins, gallo-tannins, and gallic acid esters, which results in the release of gallic acid. This enzyme can be used as a clarifying agent in the manufacture of beer, tea, wine, and juices and to treat tannin-polluting agricultural waste and industrial effluents.

Unit Definition

1 U corresponds to the amount of enzyme which changes the absorbance at 310 nm by 1.0 per minute at pH 4.7 and 30°C (tannic acid as substrate, final volume 3 ml)

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves