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Millipore

Universal Pre-Enrichment Broth

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein enzymic hydrolysate, 5.0 g/L
dextrose, 0.50 g/L
disodium phosphate, 7.0 g/L
ferric ammonium citrate, 0.10 g/L
magnesium sulfate, 0.250 g/L
monopotassium phosphate, 15.0 g/L
proteose peptone, 5.0 g/L
sodium chloride, 5.0 g/L
sodium pyruvate, 0.20 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.3±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation
microbiology

suitability

nonselective for Listeria spp.
nonselective for Salmonella spp.

Related Categories

General description

Universal Pre-enrichment Broth (UPB) is used for recovering sublethally injured Salmonella and Listeria from food products. UPB lacks inhibitory agents to provide selectivity for target pathogens and may not be adapted for samples with high levels of background microflora.

Application

Universal Pre-enrichment Broth (UPB) has been used in the enrichment of Listeria monocytogenes and Salmonella species. It has also been used in the growth kinetic studies of Salmonella typhimurium.

Preparation Note

Suspend 38.05 grams in 1000 ml distilled water. Heat if necessary, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM3714
BCCL1566
BCCK9347
BCCK3230
BCCH1949

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Ratthaphol Charlermroj et al.
International journal of food microbiology, 224, 47-54 (2016-03-08)
This study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, Campylobacter jejuni, Listeria monocytogenes, and Salmonella spp., for its actual application in routine food testing. Due to the limitation of the detection limit
Qianwang Zheng et al.
Food microbiology, 52, 159-168 (2015-09-05)
The ability of nine commercial broths to enrich healthy and 90% sanitizer-injured Salmonella Typhimurium and Salmonella cocktail on mung bean sprouts was evaluated to select an optimum broth for detection. Results showed that S. Typhimurium multiplied faster and reached a

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