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817202

TSKgel® ODS-80Ts (5 µm) HPLC Columns

L x I.D. 25 cm × 4.6 mm, C18 (octadecyl) phase

Synonym(s):

TSKgel® Reversed Phase HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

TSKgel® ODS-80Ts HPLC Column, phase C18 (octadecyl), L × I.D. 25 cm × 4.6 mm, 5 μm particle size

material

stainless steel

Agency

suitable for USP L1

product line

TSKgel®

manufacturer/tradename

Tosoh ODS-80Ts, 17202

extent of labeling

15% Carbon, monolayer, endcapped

parameter

1.2 mL/min flow rate
10-100% organic solvent
10-50 °C temperature
300 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

425 m2/g

matrix

silica particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å pore diameter

operating pH

2.0-7.5

separation technique

reversed phase

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General description

TSKgel ODS-80Ts is a general purpose column for low MW pharmaceuticals, bases, nucleosides and nucleotides. Common applications include purity checks and peptide mapping. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-80Ts has 80Å nominal pore size. The pore size of the base silica is 100Å.

Application

TSK gel ODS 80Ts HPLC column may be used for the separation of:
  • Tea catechins and quercetin conjugates in blood plasma by high-performance liquid chromatography (HPLC) coupled to electrochemical detection (ECD).
  • Active pharmaceutical ingredients (APIs) as well as their impurities in eye drop solution samples by HPLC with photodiode array (PDA) detector.
  • Drug substances by LC equipped with ultraviolet-visible (UV-Vis) detector.

TSKgel® ODS (octadecylsilane )-80Ts HPLC Column was used in HPLC analysis, done for the isolation and identification of isoflavones in blood plasma. It was also used in the measurement of quercetin glucosides in onion and in determination of quercetin metabolites in human plasma during HPLC analysis.

Physical form

Shipped in 70% methanol - 30% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Daidzein and genistein but not their glucosides are absorbed from the rat stomach.
Mariusz PK
Febs Letters, 447 (2), 287-291 (1999)
Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women
Moon JH, et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 279(2), R461-R467 (2000)
Quality by design: multidimensional exploration of the design space in high performance liquid chromatography method development for better robustness before validation
Monks K, et al.
Journal of Chromatography A, 1232(2), 218-230 (2012)
Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women.
Moon JH
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 279 (2), 461-467 (2000)
Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection
Sano M, et al.
Analyst, 126(6), 816-820 (2001)
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