51576-U
Ascentis® Express 90 Å RP-Amide (2 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm UHPLC Column
Synonym(s):
Core-shell (SPP) Fused Core polar embedded HPLC column
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
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product name
Ascentis® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L60
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
1000 bar max. pressure (14500 psi)
60 °C temp. range
technique(s)
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
120 m2/g
impurities
<5 ppm (metals)
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
amide, alkyl phase
particle size
2 μm
pore size
90 Å pore size
pH
2-9
application(s)
food and beverages
separation technique
reversed phase
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General description
Ascentis® Express 2 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on a new Fused-Core® particle design. The fused core partical provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 micron thick porous shell and the small overall particle size of 2-microns. The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.
Application
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study demonstrates the application of the Ascentis® Express RP-Amide, 2 μm UHPLC Column for analyzing complex mixtures of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The developed method was validated for precision, accuracy, and robustness, showing significant improvement in separation efficiency and analysis time compared to conventional columns. This method is particularly valuable for researchers focusing on phytochemical analysis and natural product chemistry (Fibigr et al., 2018).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
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Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
Martin Mzik et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1020, 85-89 (2016-04-01)
A simple, rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the quantitative determination in rat plasma of a new candidate for AD treatment, namely PC 48 (a 7-MEOTA-donepezil like compound) in
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
S Fan et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic
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