The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Components
The supplements contain all of the following components except as indicated: Amino acids: All standard amino acids except for histidine, leucine, tryptophan and uracil. Each amino acid is present at a concentration of 76 mg/L. Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
Plants comprise many asymptomatic fungal endophytes with potential roles of plant protection against abiotic and biotic stresses. Endophytes communicate with their host plant, with other endophytes and with invading pathogens but their language remains largely unknown. This work aims at
It says to add 1.4g to a liter of medium. Would this mean you would need to add 14g to a 10X stock solution that is suggested when preparing yeast nitrogen base without AA.
1 answer
Technical Support
·a year ago
Mathematically, yes, this would mean 14 grams would be added to a 10X stock. However, studies of the solubility of this supplement at 10X have not been performed. The end user should attempt this using a smaller volume to verify solubility. Please see the links below to the product datasheet, the Yeast Drop Out page, and the Yeast Growth Protocols page:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
