T1001

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

• Synonym(s): Globulins thyro, Thyractin
• CAS Number: 9010-34-8
• EC Number: 232-721-3
• MDL number: MFCD00132429
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine thyroid
• Assay: ≥90% (agarose gel electrophoresis)
• form: powder
• mol wt: ~670 kDa
• composition: Iodine, ≥0.20%
• nitrogen analysis: 13.0-16.0% total
• ash: ≤4%
• solubility: H₂O: 20 mg/mL
• UniProt accession no.: P01267
• storage temp.: −20°C
• Gene Information: cow ... TG(280706)

Description

Application

Bovine thyroglobulin was shown to stimulate proliferation in rat thyroid follicular cells as well as in primary porcine thyroid epithelial cells. This effect was mediated through PI3K signaling.

Often used as a carrier protein for the production of antibodies. Its advantage is the large number of tyrosine residues that can be used for conjugation using the diazo reaction.

Biochem/physiol Actions

Bovine thyroglobulin is a large, tetrameric glycoprotein produced in the thyroid gland as a precursor to thyroid hormones. Its large size and absence from plasma under normal conditions makes it very immunogenic and reduces the chance that antibodies to this carrier protein will cross-react with plasma proteins in immunoassays. Haptens may be conjugated to thyroglobulin via accessible secondary amines.

Iodine-containing protein that is stored in the thyroid gland. Converted into circulating thyroxines when the thyroid is stimulated by thyroid-stimulating hormone (TSH) from the pituitary. Because its expression is unique to the thyroid, it is a sensitive indicator of recurrence or metastasis of thyroid cancer that has been treated by thyroid ablation.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves