Stock solutions remain stable for a period of up to two weeks when stored at 4°C.
T0665
holo-Transferrin human
powder, BioReagent, suitable for cell culture, ≥97%
Synonym(s):
Siderophilin, iron-saturated
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50 MG
₹11,544.00
100 MG
₹19,291.80
500 MG
₹69,430.50
1 G
₹1,27,361.40
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50 MG
₹11,544.00
100 MG
₹19,291.80
500 MG
₹69,430.50
1 G
₹1,27,361.40
About This Item
Recommended Products
biological source
human plasma
Quality Level
description
Iron-saturated
product line
BioReagent
Assay
≥97%
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods)
endotoxin, tested
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... TF(7018)
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Application
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
Biochem/physiol Actions
Provides iron to cells in a physiologically stable and safe form.
Preparation Note
Heat treated at 60 °C (minimum) for a minimum of 10 hours.
Analysis Note
Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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What is the recommended storage method for stock solutions of item T0665, holo-Transferrin human?
1 answer-
Helpful?
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What's the process of saturation? Do the cells express directly holo-Tf with iron? Or apo-Tf reacts with iron salts?
1 answer-
The delivery of iron to cells by transferrins is a receptor-mediated and controlled process. Cells regulate the amount of iron they receive from the extracellular environment by varying transferrin receptor expression. Two iron-loaded transferrin molecules bind to one transferrin receptor. Once bound, they are transferred into acidic endosomes within the cell’s interior. At reduced pH, the iron is released and incorporated into intracellular proteins and the storage molecule ferritin. The apo-transferrin (iron-free) is recycled back to the exterior of the cell and released. Each transferrin receptor cycle can deliver up to four atoms of iron.
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Holo-Tf is described as "iron saturated", but there is a statement "Under normal circumstances, approximately one-third of transferrin iron-binding pockets are filled." I'm confused if the product I got (T0665) is 100% saturated with iron or not.
1 answer-
This product is a Holo-Transferrin form which is generally considered to be iron-saturated. The specification for iron content of this product is 0.10 - 0.35%. Conversely, Apo-Transferrin is considered to be iron deficient. Apo-transferrin product T5391 has an iron content specification of <0.005%. Each Transferrin has the capacity to bind 2 iron (Fe3+) molecules. In a circulating system, the iron content is transitory and rarely (if ever) 100% saturated. This product is tested for total iron content. The number of binding sites that are filled is not measured. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/520/t0665pis.pdfHelpful?
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What is the recommended holo-transferrin solvent and how should the dissolved protein be stored?
1 answer-
This product is tested for solubility in water at 50 mg/mL. Rates are expected to be comparable when dissolving in an aqueous buffer or medium. Solutions are stable for 5-10 days at 2-8°C. This product has not been evaluated for long-term solution stability. However, medium supplements containing transferrin are stable when stored in aliquots at -20°C for up to one year. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdfHelpful?
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What is the carbohydrate content of human transferrin?
1 answer-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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